Fig. 3

Expression profiles of the derived goat iPSCs. a the properties of goat iPS1 cell line were evaluated with ESCs markers, including OCT4, SOX2, KLF 4, NANOG, CDX2, REX, SSEA-1, TRA-1-60 and TRA-1-81. All these markers were positively stained in the goat iPS cell line. GEFs without transfections were used as the blank control, which did not express ESCs markers. Immunofluorescence staining of goat iPS1 cells without primary antibodies was used as the negative control. Scale bar: 50 μm. b qRT-PCR analysis confirmed that the goat iPS1 cell line express a variety of ESCs marker genes, including Oct4, Sox2, Nanog, Dax1 and Gdf3. The expression of DNA methyltranferase Dnmt3b and DNA dymethyltransferase TET1, 2, 3 were also detected with qRT-PCR. c Quantitative evaluation of Oct4, Sox2, and Nanog expression in different passages of goat iPS1 cell line. Up: Oct4, Middle: Sox2, Down: Nanog