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Fig. 2 | BMC Biotechnology

Fig. 2

From: Inducing goat pluripotent stem cells with four transcription factor mRNAs that activate endogenous promoters

Fig. 2

Dynamic changes of the GEFs morphology during goat iPSCs induction. a The schematic diagram of mRNA induced goat iPSCs induction process. b GEFs morphology continuously changed during iPSCs induction. GEFs without transfection were shown in the shape of spindle. Six days after mRNA transfection, the culturing medium was changed to iPSCs induction medium, and the GEFs became round. On day 10, the cells exhibited aggregation growth feature. On day 15, small clone-like cells appeared. On day 21, goat ESC-like clones with clear cell borders were observed, which were positively stained with AKP. Scale bar: 50 μm

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BMC Biotechnology

ISSN: 1472-6750

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