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Table 3 Oligonucleotides used in this study
| Name | Sequence (5’➔3’) | Specificity |
|---|---|---|
| 3’pcbC_s | TTCGTCGAGAACGGTGAAGC | downstream region of pcbC 3’flank used for homologous recombination |
| Phleo_s | TCCTGCGCCTGATACAGAAC | 3’region phleomycin resistance cassette |
| PtrpC_a | TGTTGACCTCCACTAGCTCC | 5’region PtrpC |
| 5’pcbC_a | TAGTGGCCGAGAAGCCTATC | upstream region of pcbC 5’ flank used for homologous recombination |
| pcbC_s | TATACCATGGATGGCTTCCACCCCCAAGG | pcbC |
| pcbC_a | TACTCCATGGTAGTCTGGCCGTTCTTGTTG | pcbC |
| SSU1 | ATCCAAGGAAGGCAGCAGGC | SSUr RNA |
| SSU2 | TGGAGCTGGAATTACCGCG | SSUr RNA |
| PcpcbC-RT_s | CCCTCCCGTTCTTCGTCAATC | pcbC (qRT-PCR) |
| PcpcbC-RT_a | CTGCAGATAGTAGCCGTACGA | pcbC (qRT-PCR) |
