Table 2 Plasmids used in this study
Plasmid | Characteristics | Source |
|---|---|---|
pD-Phleo | trpC(p)::ble | [46] |
pKOvelB | 1 kb 5’ flank region and 1 kb 3’ flank region of PcvelB with FRT sites in pD-Phleo | This study |
pKOpcbCFRTble | Replacement of PcvelB-specific flanks by pcbC-specific flanks in pKOvelB | This study |
pKOvosA | 5’PcvosA flank, 5’FRT, PtrpC, nat1 resistance gene, 3’FRT sequence, 3’PcvosA flank | [16] |
pPTRII_PcFLP | trpC promoter, Pcflp gene, ptrA resistance gene of A. oryzae, AMA1 sequences of A. nidulans | [16] |
pKOpcbCFRTnat | Replacement of PcvosA-specific flanks by pcbC-specific flanks in pKOvosA | This study |
ppcbCFRTnat | Introduction of pcbC ORF behind 5’ pcbC flank in pKOpcbCFRTnat | This study |
pGFP-pcbC | Pgpd::pcbC::egfp::Ttrpc, PtrpC::nat1 | This study |
pPCPV1 | Plasmid harbouring the complete penicillin biosynthesis cluster and the niaD gene | Kamerewerd and Kück, unpublished |