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Fig. 4 | BMC Biotechnology

Fig. 4

From: Penicillin production in industrial strain Penicillium chrysogenum P2niaD18 is not dependent on the copy number of biosynthesis genes

Fig. 4

Generation of pcbC complementation strains by complementation with the complete penicillin biosynthesis cluster. The pcbC null mutant was complemented with plasmid pPCPV1 harboring the complete biosynthesis cluster (a). PFGE proved that integration of the plasmid occurred in the genomic region of the penicillin cluster and further showed single (T1, T4) or double (T2, T3) integration of the plasmid (b). The gene content of individual strains is indicated above each lane, for comparison strain Δku70 (ΔPcku70 EK2) is given

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