TY - JOUR AU - Ziemons, Sandra AU - Koutsantas, Katerina AU - Becker, Kordula AU - Dahlmann, Tim AU - Kück, Ulrich PY - 2017 DA - 2017/02/16 TI - Penicillin production in industrial strain Penicillium chrysogenum P2niaD18 is not dependent on the copy number of biosynthesis genes JO - BMC Biotechnology SP - 16 VL - 17 IS - 1 AB - Multi-copy gene integration into microbial genomes is a conventional tool for obtaining improved gene expression. For Penicillium chrysogenum, the fungal producer of the beta-lactam antibiotic penicillin, many production strains carry multiple copies of the penicillin biosynthesis gene cluster. This discovery led to the generally accepted view that high penicillin titers are the result of multiple copies of penicillin genes. Here we investigated strain P2niaD18, a production line that carries only two copies of the penicillin gene cluster. SN - 1472-6750 UR - https://doi.org/10.1186/s12896-017-0335-8 DO - 10.1186/s12896-017-0335-8 ID - Ziemons2017 ER -