Optimization of laccase production from Marasmiellus palmivorus LA1 by Taguchi method of Design of experiments

BMC Biotechnology

Table 4 Analysis of variance (ANOVA)

Sl.No:	Factors	DOF	Sums of squares	Variance	F-Ratio	Pure Sum	Percent
1	Temperature (°C)	1	0.006	0.006	173.585	0.006	0.060
2	pH	1	0.053	0.053	1,325.232	0.053	0.464
3	NH₄H₂PO₄ (%)	1	0		POOLED	0	0
4	Galactose (%)	1	0.401	0.401	10,006.075	0.401	3.510
5	Cupric sulphate (mM)	1	8.366	8.366	208,581.269	8.366	73.181
6	Inoculum concentration	1	0.108	0.108	2,694.782	0.108	0.945
7	Substrate length (m)	1	2.496	2.496	62,237.074	2.496	21.835
	Other/Error	1	0	0			0.005
	Total	7	11.433				100.00%

ISSN: 1472-6750