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Table 3 Predicted interactions of the given factors depicted via severity index

From: Optimization of laccase production from Marasmiellus palmivorus LA1 by Taguchi method of Design of experiments

Sl. No:

Interacting factor pairs (Order based on SI)

Columns

SI (%)

Col.

Opt.

1

NH4H2PO4 (%) x Inoculum concen.

3 × 6

89.72

5

[1,2]

2

Temperature (°C) x Inoculum concen.

1 × 6

82.66

7

[1,2]

3

Temperature (°C) x Galactose (%)

1 × 4

82.06

5

[2,1]

4

Temperature (°C) x NH4H2PO4 (%)

1 × 3

76.01

2

[2,1]

5

NH4H2PO4 (%) x Galactose (%)

3 × 4

71.42

7

[2,1]

6

pH x Inoculum concen.

2 × 6

65.61

4

[2,2]

7

pH x Substrate length (m)

2 × 7

64.67

5

[1,2]

8

pH x Cupric sulphate (mM)

2 × 5

35.32

7

[1,2]

9

Substrate length (m) x Galactose (%)

2 × 4

34.38

6

[2,1]

10

NH4H2PO4 (%) x Substrate length (m)

3 × 7

28.57

4

[2,2]

11

pH x NH4H2PO4 (%)

2 × 3

23.98

1

[2,1]

12

Galactose (%) x Inoculum concen.

4 × 6

23.92

2

[1,2]

13

Temperature (°C) x Cupric sulphate (mM)

1 × 5

17.93

4

[2,2]

14

Temperature (°C) x Substrate length (m)

1 × 7

17.33

6

[1,2]

15

NH4H2PO4 (%) x Cupric sulphate (mM)

3 × 5

10.27

6

[1,2]

16

Cupric sulphate (mM) x Substrate length (m)

5 × 7

5.15

2

[2,2]

17

Inoculum concen. x Substrate length

6 × 7

3.8

1

[2,2]

18

Temperature (°C) x pH

1 × 2

2.08

3

[2,2]

19

Galactose (%) x Cupric sulphate (mM)

4 × 5

2.06

1

[1,2]

20

Galactose (%) x Substrate length (m)

4 × 7

.28

3

[1,2]

21

Cupric sulphate (mM) x Inoculum concen.

5 × 6

.19

3

[2,2]

  1. Columns: Column locations to which the interacting factors are assigned, SI%: Interaction severity index, Col: Column that should be reserved if this particular interaction is to be studied, Opt.: indicates factor levels desirable for the optimum condition