Fig. 6

RNAi of Smad1/5 reduces RA-mediated induction of germ cell-specific gene expression. a Smad1 and Smad5 mRNA expression levels in siRNA-infected cBCs were determined by qRT-PCR and semi-quantitative PCR. Values are the mean ± SEM (n = 3) and each condition is normalized to the abundance of β-actin as determined by qRT-PCR. b After co-infecting Smad1 and Smad5 siRNA, the mRNA expression levels of Stra8, Dazl and Cvh were measured in the absence or presence of RA. Data are representative of results in three independent experiments, values are the mean ± SEM (n = 3) and each condition is normalized to β-actin abundance. *, p < 0.05; **, p < 0.01