Fig. 1From: Retinoic acid promotes expression of germline-specific genes in chicken blastoderm cells by stimulating Smad1/5 phosphorylation in a feeder-free culture systemThe growth of chicken blastodermal cells (cBCs) in E8 medium. a The cells have large nuclei and pronounced nucleoli (arrows), and grow in a monolayer with clearly distinguishable individual cells. b The cells form tight and compact colonies of multilayer cells arranged in clusters after FGF9 and TGF-β are withdrawn. c Immunofluorescence staining of SSEA-1 (green), nuclei were counterstained with DAPI (blue)Back to article page