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Fig. 5 | BMC Biotechnology

Fig. 5

From: Automated freeze-thaw cycles for decellularization of tendon tissue - a pilot study

Fig. 5

Fluorescence microscopic assessment of cytocompatibility. Representative panels of LIVE/DEAD® staining of equine superficial digital flexor tendon specimens decellularized by automated (a) and manual (b) freeze thaw cycles. Decellularized scaffolds were re-seeded with equine adipose tissue derived mesenchymal stromal cells and a fluorescence microscopic evaluation was performed after 3 days of culture. Vital cells are indicated by green fluorescence (display of intracellular esterase activity), cells with defect cellular membranes show a red fluorescence signal of their nucleus

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