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Fig. 5 | BMC Biotechnology

Fig. 5

From: Knockdown of Mythimna separata chitinase genes via bacterial expression and oral delivery of RNAi effectors

Fig. 5

Validation for oral ingestion and molecular evidence for RNAi effects. a Photograph of M. separata larva feeding on the artificial diet. b Gut tissue from the larvae fed on the artificial diet mixed with (upper row) or without (lower row) FD&C Blue. c The same experiment with (b) where the intestinal content was removed from the gut tissues. d and (e) Knockdown of M. separata chitinase gene after feeding on the bacteria expressing dsRNAs. T-test was performed between treatment and control group. The single asterisk indicates a significant difference at p < 0.05. f and (g) Northern blot detection of small RNA in the larvae fed on dsRNA-expressing bacteria. f Lane 1, Larvae fed on the bacteria expressing MseChi1 dsRNA; Lane 2, Larvae fed on the bacteria transformed with empty L4440 vector. g Lane 1, Larvae fed on the bacteria expressing MseChi2 dsRNA; Lane 2, Larvae fed on the bacteria transformed with empty L4440 vector

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