Fig. 5From: Knockdown of Mythimna separata chitinase genes via bacterial expression and oral delivery of RNAi effectorsValidation for oral ingestion and molecular evidence for RNAi effects. a Photograph of M. separata larva feeding on the artificial diet. b Gut tissue from the larvae fed on the artificial diet mixed with (upper row) or without (lower row) FD&C Blue. c The same experiment with (b) where the intestinal content was removed from the gut tissues. d and (e) Knockdown of M. separata chitinase gene after feeding on the bacteria expressing dsRNAs. T-test was performed between treatment and control group. The single asterisk indicates a significant difference at p < 0.05. f and (g) Northern blot detection of small RNA in the larvae fed on dsRNA-expressing bacteria. f Lane 1, Larvae fed on the bacteria expressing MseChi1 dsRNA; Lane 2, Larvae fed on the bacteria transformed with empty L4440 vector. g Lane 1, Larvae fed on the bacteria expressing MseChi2 dsRNA; Lane 2, Larvae fed on the bacteria transformed with empty L4440 vectorBack to article page