Fig. 4

Northern hybridization detection of dsRNA produced in HT115 cells. a Resolution of DIG-labelled probes on agarose gel. MseChi1 or MseChi2 partial sequences were labelled by modified PCR with the addition of 0.05 mM of DIG-11-dUTP. DIG-labelled probes were electrophoresed on 1% agarose gel. Northern blot detection of MseChi1 (b) or MseChi2 (c) dsRNA after IPTG induction in HT115 cells. The cell cultures were processed for total RNA extraction. The RNA samples were resolved on 1% agarose gel after glyoxal denaturation. RNA was then transferred to nylon membranes and fixed using the crosslinker. Hybridization was carried out and the hybridized probes were finally visualized with BCIP/NBT Alkaline Phosphatase Substrate Solution