Fig. 3From: Knockdown of Mythimna separata chitinase genes via bacterial expression and oral delivery of RNAi effectorsConfirmation of dsRNA produced in HT115 cell. The recombinant plasmids were transformed into HT115 competent cell. Individual transformant was cultured on 2 X YT media with or without addition of IPTG. The cell cultures were processed for total RNA extraction. RNA samples were resolved on 1% agarose gel before (a) or after (b) treatment with RNaseA. Arrowhead indicates the position of dsRNA bandBack to article page