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Fig. 2 | BMC Biotechnology

Fig. 2

From: Knockdown of Mythimna separata chitinase genes via bacterial expression and oral delivery of RNAi effectors

Fig. 2

Schematics of the nucleotide fragments for different experiments. a Detail of the nucleotide regions used in the study for MseChi1 gene. Red line indicates the region for dsRNA synthesis, purple line indicates the region for preparation of DIG-labelled probe, green line indicates the region for amplification of the fragment for semi-quantitative RT-PCR analysis, and blue line indicates the region for amplification of the fragments in qRT-PCR experiment. b Detail of the nucleotide regions used in the study for MseChi2 gene. c General scheme for dsRNA production by L4440 vector in HT115 cell

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