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Fig. 1 | BMC Biotechnology

Fig. 1

From: Knockdown of Mythimna separata chitinase genes via bacterial expression and oral delivery of RNAi effectors

Fig. 1

Phylogenetic analysis and expression patterns of M. separata chitinase genes. a Phylogenetic analysis of MseChi1 and MseChi2 by aligning with putative chitinase and chitinase-like protein encoding genes from other insect species using MEGA 6.0 software. Abbreviations, Mse: Mythimna separata. Mb: Mamestra brassicae. Se: Spodoptera exigua. Sl: Spodoptera litura. Of: Ostrinia furnacalis. Px: Papilio xuthus. Sc: Samia cynthia. Ms: Manduca sexta. Ac: Agrius convolvuli. Bm: Bombyx mori. Sf: Spodoptera frugiperda. Ha: Helicoverpa armigera. Ai: Agrotis ipsilon. Mc: Mamestra configurata. Lo: lacanobia oleracea. denotes M. separata chitinase genes. b Expression profiles of MseChi1 and MseChi2 in a variety of tissues including reproductive organ (RO), fat body (FB), thorax (T), head (H), abdomen (A) and gut (G). Semi-quantitative RT-PCR was performed for MseChi1 and MseChi2 using the gene specific primers at 32 cycles. 28 cycles of amplification for the house-keeping gene β-actin was carried out to test the integrity of tissue sample

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