Fig. 1

Bias plots, showing percentage of observed bases at each position (or sequencing cycle). Plots show insertion bias for standard Tn5 in NexteraV2 kit and two Tn5 mutants from sequencing E. coli genomic DNA. The intensities after position (cycle) 20 are the base composition of E. coli genomic DNA. a Standard Tn5 in NexteraV2 kit. Notice the symmetry in the plot centered at position 5, between positions 1 through 9 b Tn5 mutant, notice the change in the insertion bias at positions 3 through 7 and position 12 c Tn5 mutant, notice the change in the insertion bias at positions 3 through 7, as well as higher G bias at position 1