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Fig. 2 | BMC Biotechnology

Fig. 2

From: New ligation independent cloning vectors for expression of recombinant proteins with a self-cleaving CPD/6xHis-tag

Fig. 2

Purification strategy of autoprocessing CPD fusion-tag. a Self-cleaving CPD-fusion protein purification; b Purification of MLD. Marker (M), Total proteins (Tot), Soluble proteins (Sol), Washing step with 50 mM imidazole (W), Elution step with 500 mM imidazole containing MLD-CPD (E1), 1 h incubation with InsP6 (InsP6), First flow-through fraction containing MLD (FT1), Second flow-through fraction (FT2), Elution step with 500 mM imidazole containing CPD (E2), and previously purified MLD (MLD)

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BMC Biotechnology

ISSN: 1472-6750

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