Fig. 1

Design and cloning strategy of pCPD. a Sequence of LIC site in pMCSG58; b Sequence of LIC site in pCPD; c Molecular cloning strategy for the pCPD vector. After SmaI digestion linearized pCPD is treated with T4 polymerase and dATP. Target protein is amplified by PCR using LIC primers. PCR product is treated with T4 polymerase and dTTP. Both vector and PCR product are mixed for annealing and then transformed into competent cells