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Table 1 Analysis of isolated antigen-specific B cells

From: Antigen-specific single B cell sorting and expression-cloning from immunoglobulin humanized rats: a rapid and versatile method for the generation of high affinity and discriminative human monoclonal antibodies

Targeted antigen Number of analyzed wells Number of wells with HC gamma amplification Number of wells with HC and LC associated (% recovery) Mutations in variable HC and LC Number of antigen-specific produced antibodies Affinity determined by SPR (KD)
β-galactosidase 14 12 8 (57%) Yes 8 1 × 10−10 to 1 × 10−7M
Fc-CD22 30 6 6 (20%) Yes 1 anti-CD22; 2 anti-Fc 1,6 × 10−9M
HLA-A 7 6 6 (86%) Yes 1 1,27 × 10−8M
HLA-A2/Pp65 24 11 9 (38%) Yes 3 6.10−8 to 1,4.10−7M
CD22 72 49 44 (61%) Yes 27 Not determined