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Fig. 3 | BMC Biotechnology

Fig. 3

From: Antigen-specific single B cell sorting and expression-cloning from immunoglobulin humanized rats: a rapid and versatile method for the generation of high affinity and discriminative human monoclonal antibodies

Fig. 3

Characterization of a HLA-A2/Pp65 specific mAb (PC1.02) isolated from human peripheral blood. a Specificity of mAbs by ELISA. Plates were coated with relevant (HLA-A2/Pp65) or irrelevant HLA-A2 complexes containing HLA-A2-restricted peptides: MelA, NS3 (HCV-1) and GagP17 (HIV-1) at 2 μg/mL, the mAb PC1.02 was added and an anti-human IgG-HRP Ab was used for detection. OD were read at 450 nm. b Affinity determination of the mAb PC1.02 using SPR by flowing various concentration of A2/Pp65 complexes over CM5 chip-bound mAb

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