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Fig. 2 | BMC Biotechnology

Fig. 2

From: Antigen-specific single B cell sorting and expression-cloning from immunoglobulin humanized rats: a rapid and versatile method for the generation of high affinity and discriminative human monoclonal antibodies

Fig. 2

Overall strategy of reconstruction of recombinant human mAbs. A tetramer-based sorting strategy allows detection of B cells of interest. Tetramer+ B cells were single cell sorted. Light and heavy chain variable encoding segments were amplified using RT-PCR. Antibody genes PCR products were sequenced directly and cloned into separate eukaryotic expression vectors in frame with constant light and heavy regions genes. Expression of the corresponding fully human mAbs by transiently-transfected HEK 293 cells was purified from the culture supernatant

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