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Fig. 1 | BMC Biotechnology

Fig. 1

From: Antigen-specific single B cell sorting and expression-cloning from immunoglobulin humanized rats: a rapid and versatile method for the generation of high affinity and discriminative human monoclonal antibodies

Fig. 1

Detection of HLA-A2/Pp65 specific B cells from human PBMC. a Gating strategy for HLA-A2/Pp65 specific B cells. After immunomagnetic enrichment with HLA-A2/Pp65 tetramers and staining with additional fluorescent mAbs, cells were analyzed by flow cytometry. Cells were gated first on viable singlet lymphocytes, then on CD19+CD3− cells. B cells that stained with both HLA-A2/Pp65-PE and HLA-A2/Pp65-APC tetramers were gated. Cells were also stained with HLA-A2/irrelevant peptides to exclude B cells that were not against HLA-A2/Pp65. b The frequency of HLA-A2/MelA (n = 36), HLA-A2/Pp65 (n = 37), HLA-A2/GagP17 (n = 28) specific B cells was determined among total peripheral B cells in healthy donors

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BMC Biotechnology

ISSN: 1472-6750

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