TY - JOUR AU - Ouisse, Laure-Hélène AU - Gautreau-Rolland, Laetitia AU - Devilder, Marie-Claire AU - Osborn, Michael AU - Moyon, Melinda AU - Visentin, Jonathan AU - Halary, Frank AU - Bruggemann, Marianne AU - Buelow, Roland AU - Anegon, Ignacio AU - Saulquin, Xavier PY - 2017 DA - 2017/01/09 TI - Antigen-specific single B cell sorting and expression-cloning from immunoglobulin humanized rats: a rapid and versatile method for the generation of high affinity and discriminative human monoclonal antibodies JO - BMC Biotechnology SP - 3 VL - 17 IS - 1 AB - There is an ever-increasing need of monoclonal antibodies (mAbs) for biomedical applications and fully human binders are particularly desirable due to their reduced immunogenicity in patients. We have applied a strategy for the isolation of antigen-specific B cells using tetramerized proteins and single-cell sorting followed by reconstruction of human mAbs by RT-PCR and expression cloning. SN - 1472-6750 UR - https://doi.org/10.1186/s12896-016-0322-5 DO - 10.1186/s12896-016-0322-5 ID - Ouisse2017 ER -