Table 1 Primer sets used in the plasmid construction
Primer | Target | aSequence | Sense | Product |
|---|---|---|---|---|
HA-SpeI-F | H7/HA | GCCTAGACTAGTATGAACACTCAAATCCTG | + | 1683Â bp |
HA-KpnI-R | H7/HA | GCACGGTACCTTATATACAAATAGTGCACC | - | |
NA-BamHI-F | N9/NA | TAGATCGGATCCATGAATCCAAATCAGAAG | + | 1398Â bp |
NA-KpnI-R | N9/NA | GATCGGTACCTTAGAGGAAGTACTCTATTT | - | |
M1-BamHI-F | M1 | TAGATCGGATCCATGAGTCTTCTAACCGAG | + | 759Â bp |
M1-HindIII-R | M1 | AGCTAAGCTTTCACTTGAACCGCTGCAGTT | - |