Skip to main content
Fig. 4 | BMC Biotechnology

Fig. 4

From: Multi-antigen avian influenza a (H7N9) virus-like particles: particulate characterizations and immunogenicity evaluation in murine and avian models

Fig. 4

The immunogenicity of H7N9 VLP in a mouse model. a Mice (n = 6 per group) were immunized with VLPs (10 μg per mouse intramuscularly on day 0 and 14) or PBS, and blood samples were collected. Mice receiving H7N9 VLPs showed significantly increased serum HI titer against the H7N9 inactivated virions (b) or H7N9 VLPs (c). Mice splenocytes were isolated on day 28 and restimulated ex vivo with the H7N9 VLPs. The antigen-specific cytokine responses were detected by intracellular cytokine staining. Comparing with the PBS control, mice receiving the H7N9 VLPs showed elevated CD4 IL-4 and CD8 TNF production (d, f), whereas the production of CD4 IFN-γ or CD8 IFN-γ revealed no statistical difference (e, g). Error bars are mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001

Back to article page