Fig. 3From: Multi-antigen avian influenza a (H7N9) virus-like particles: particulate characterizations and immunogenicity evaluation in murine and avian modelsThe formation of VLP and proper antigen display of viral proteins were verified. a The HA (left) and NA (right) proteins were detected by antibodies labeled with 6 nm gold under a transmission electron microscope. Scale bars = 50 nm. b Size distribution and (c) surface zeta potential of the VLPs were analyzed using nanoparticle tracking analysis. Influenza virus A/PuertoRico/8/34(H1N1) was analyzed under the same settings as a reference. d Hemagglutination activity of H7N9 VLPs was assessed by a hemagglutination assay. The total protein contents of H7N9 VLP are indicated based on a two-fold serial dilution. PBS was included as the negative controlBack to article page