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Fig. 3 | BMC Biotechnology

Fig. 3

From: Multi-antigen avian influenza a (H7N9) virus-like particles: particulate characterizations and immunogenicity evaluation in murine and avian models

Fig. 3

The formation of VLP and proper antigen display of viral proteins were verified. a The HA (left) and NA (right) proteins were detected by antibodies labeled with 6 nm gold under a transmission electron microscope. Scale bars = 50 nm. b Size distribution and (c) surface zeta potential of the VLPs were analyzed using nanoparticle tracking analysis. Influenza virus A/PuertoRico/8/34(H1N1) was analyzed under the same settings as a reference. d Hemagglutination activity of H7N9 VLPs was assessed by a hemagglutination assay. The total protein contents of H7N9 VLP are indicated based on a two-fold serial dilution. PBS was included as the negative control

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