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Fig. 2 | BMC Biotechnology

Fig. 2

From: Multi-antigen avian influenza a (H7N9) virus-like particles: particulate characterizations and immunogenicity evaluation in murine and avian models

Fig. 2

Expression and characterization of H7N9 VLP. a The viral titers of three recombinant baculoviruses were determined by plaque assay. The titer of recombinant baculovirus-HA (rBac-HA) was approxmiately 6 × 106 PFU/ml (left), the rBac-NA 3 × 107 PFU/ml (center), and the rBac-M1 1.1 × 108 PFU/ml (right). The protein expression of HA, NA, and M1 was identified respectively by immunofluorescence assay (lower panel). b Sf9 cells were co-infected with rBac-HA, rBac-NA, and rBac-M1 under various MOI combinations, and the VLPs were purified via sucrose gradient centrifugation. Formulation derived from the combinations of rBac-HA (MOI 2), rBac-NA (MOI 2), and rBac-M1 (MOI 2) co-infection exhibited the highest HA activity. c H7N9 VLP, H7/HA protein, N9/NA protein, and M1 proteins were detected by Western blot

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BMC Biotechnology

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