Fig. 5
Biological characterization of isolated hMSCs from acoustic stimulated BM at 300 and 500Hz for 5 and 10 min. The results are presented as the fold change over the non-stimulated BM (baseline). a Proliferation of hMSCs calculated as PD/day from P1 to P2, donor and stimulation dependent. b Proliferation average. c CFU potential of hMSCs, donor and stimulation dependent. d CFU average. e ECM production, quantification of nodule size area in mm2, donor and stimulation dependent. f ECM production average. g Osteogenic potential calculated as percentage of ALP positive colonies within the CFUs, donor and stimulation dependent. h Osteogenesis average. i Adipogenic potential, quantification of Oil red O staining relative to 100% Oil red O staining solution, donor and stimulation dependent. j Adipogenesis average. Values are represented as mean ± standard deviation of at least three independent experiments (n ≥ 3). Statistically significant differences were found with ***p < 0.001, **p < 0.01 and *p < 0.05