Fig. 3
Biological characterization of hMSCs isolated from different BM locations. a Proliferation of hMSCs calculated as PD/day from P1 to P2, donor and location dependent. b Proliferation average for all the donors. c CFU potential of hMSCs, donor and location dependent. d CFU average for all the donors. e ECM production, quantification of nodule size area in mm2 after cell condensation, donor and location dependent. f ECM production average. g Osteogenic potential calculated as percentage of ALP positive colonies within the CFUs, donor and location dependent. h Osteogenesis average. i Adipogenic potential, quantification of Oil red O staining relative to 100% Oil red O staining solution, donor and location dependent. j Adipogenesis average. The uniform distribution of data, to test inter-donor variation, was assessed using Chi-squared test and presented as a line above all donors. Values are represented as mean ± standard deviation of at least three independent experiments (n ≥ 3). Statistically significant differences were found with ***p < 0.001, **p < 0.01 and *p < 0.05