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Table 8 Summary of the data from DLS and FPLC for refolded and soluble MnP

From: Expression of a fungal manganese peroxidase in Escherichia coli: a comparison between the soluble and refolded enzymes

Size distribution

Radius (nm)

Molecular weight (kDa)

Mass (%)

Specific activity (U/mg)

Rz (Soret/280)

refolded MnP

Particle 1

2.9 ± 0.5

42.0

99.6

15.0 ± 2.1

3.1 ± 0.6

soluble MnP peak I

Particle 1

12.9 ± 0.7

1314.0

70.9

0.6 ± 1.5

1.0 ± 0.3

Particle 2

45.3 ± 1.4

25199.0

22.3

soluble MnP peak II

Particle 3

4.5 ± 0.8

111.0

99.3

0.2 ± 0.8

0.3 ± 1.1

  1. “Particle 1” of refolded MnP was presented in Fig. 7a; “Particle 1”, “Particle 2” and “Particle 3” of soluble MnP were presented in Fig. 7b and Fig. 7c. The results of hydrodynamic radius, specific activity and Rz were given as the mean ± standard deviation, calculated from at least three repetitions
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BMC Biotechnology

ISSN: 1472-6750

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