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Table 7 Substrate specificities of refolded and soluble MnP

From: Expression of a fungal manganese peroxidase in Escherichia coli: a comparison between the soluble and refolded enzymes

Substrate

Concentration (mM)

Emax (M−1 cm−1)

Wavelength (nm)

Refolded MnP (U L−1)

Soluble MnP (U L−1)

Mn2+ present

Mn2+ absent

Mn2+ present

Mn2+ absent

ABTS

1

36000

420

344.93 ± 0.03

37.73 ± 0.01

310.0 ± 0.046

24.4 ± 0.006

DMP

1

49600

469

105.42 ± 0.04

0.32 ± 0.001

104.35 ± 0.05

0

Guaiacol

1

12100

456

122.31 ± 0.003

2.27 ± 0.001

112.40 ± 0.008

0