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Table 7 Substrate specificities of refolded and soluble MnP

From: Expression of a fungal manganese peroxidase in Escherichia coli: a comparison between the soluble and refolded enzymes

Substrate Concentration (mM) Emax (M−1 cm−1) Wavelength (nm) Refolded MnP (U L−1) Soluble MnP (U L−1)
Mn2+ present Mn2+ absent Mn2+ present Mn2+ absent
ABTS 1 36000 420 344.93 ± 0.03 37.73 ± 0.01 310.0 ± 0.046 24.4 ± 0.006
DMP 1 49600 469 105.42 ± 0.04 0.32 ± 0.001 104.35 ± 0.05 0
Guaiacol 1 12100 456 122.31 ± 0.003 2.27 ± 0.001 112.40 ± 0.008 0
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