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Table 6 Kinetic parameters of refolded and soluble MnP

From: Expression of a fungal manganese peroxidase in Escherichia coli: a comparison between the soluble and refolded enzymes

Enzyme Km (Mn2+) μmol L−1 Vmax (Mn2+) μmol L−1 min−1 Kcat (Mn2+) s−1 Kcat/Km (Mn2+) (mmol)−1 L s−1
Refolded MnP 148 ± 7.1 1276 ± 23.3 141.9 ± 2.62 930 ± 30
Soluble MnP 336 ± 8.0 236.6 ± 24.7 25.6 ± 2.7 76 ± 9.9
Native MnP 100.7 ± 0.87 1116.3 ± 18.3 969 ± 22.6 9620 ± 420
Enzyme Km (H2O2) μmol L−1 Vmax (H2O2) μmol L−1 min−1 Kcat (H2O2) s−1 Kcat/Km (H2O2) (mmol)−1 L s−1
Refolded MnP 234.5 ± 12 3326.5 ± 179 554.4 ± 29 2370 ± 7
Soluble MnP 23.5 ± 4.9 308.1 ± 26.9 19 ± 1.7 820 ± 100
Native MnP 30.7 ± 4.4 1844.5 ± 25 1601.1 ± 34.8 52200 ± 450
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