Table 6 Kinetic parameters of refolded and soluble MnP
Enzyme | Km (Mn2+) μmol L−1 | Vmax (Mn2+) μmol L−1 min−1 | Kcat (Mn2+) s−1 | Kcat/Km (Mn2+) (mmol)−1 L s−1 |
|---|---|---|---|---|
Refolded MnP | 148 ± 7.1 | 1276 ± 23.3 | 141.9 ± 2.62 | 930 ± 30 |
Soluble MnP | 336 ± 8.0 | 236.6 ± 24.7 | 25.6 ± 2.7 | 76 ± 9.9 |
Native MnP | 100.7 ± 0.87 | 1116.3 ± 18.3 | 969 ± 22.6 | 9620 ± 420 |
Enzyme | Km (H2O2) μmol L−1 | Vmax (H2O2) μmol L−1 min−1 | Kcat (H2O2) s−1 | Kcat/Km (H2O2) (mmol)−1 L s−1 |
Refolded MnP | 234.5 ± 12 | 3326.5 ± 179 | 554.4 ± 29 | 2370 ± 7 |
Soluble MnP | 23.5 ± 4.9 | 308.1 ± 26.9 | 19 ± 1.7 | 820 ± 100 |
Native MnP | 30.7 ± 4.4 | 1844.5 ± 25 | 1601.1 ± 34.8 | 52200 ± 450 |