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Table 5 Isolation and purification of MnP from inclusion bodies

From: Expression of a fungal manganese peroxidase in Escherichia coli: a comparison between the soluble and refolded enzymes

Sample

Protein concentration (mg/ml)

Protein (mg)

Specific activity (U/mg)

Total activity (U)

Yield (%)

Inclusion body

18.7

93.5

   

Dialyzed

0.43

12.35

3.04

37.5

100

Ni-NTA

0.076

2.26

12.9

29.15

78