Skip to main content

Table 2 Effect of six factors on specific activity of refolded MnP

From: Expression of a fungal manganese peroxidase in Escherichia coli: a comparison between the soluble and refolded enzymes

Experiment

A

Urea

(M)

B

CaCl2

(mM)

C

Hemin

(μM)

D

Glycerol

(%)

E

GSSG

(mM)

F

pH

Specific activity

(U/mg)

1

1

1

1

1

1

1

1.63 ± 0.23

2

1

2

2

2

2

2

1.89 ± 0.59

3

1

3

3

3

3

3

0.65 ± 0.02

4

2

1

1

2

2

3

0.86 ± 0.15

5

2

2

2

3

3

1

0.86 ± 0.04

6

2

3

3

1

1

2

3.63 ± 0.19

7

3

1

2

1

3

2

2.04 ± 0.24

8

3

2

3

2

1

3

1.10 ± 0.06

9

3

3

1

3

2

1

1.01 ± 0.25

10

1

1

3

3

2

2

2.23 ± 0.39

11

1

2

1

1

3

3

1.83 ± 0.25

12

1

3

2

2

1

1

3.38 ± 0.06

13

2

1

2

3

1

3

1.02 ± 0.23

14

2

2

3

1

2

1

0.86 ± 0.07

15

2

3

1

2

3

2

2.03 ± 0.10

16

3

1

3

2

3

1

0.96 ± 0.12

17

3

2

1

3

1

2

1.52 ± 0.36

18

3

3

2

1

2

3

1.16 ± 0.09

K1

11.61

8.742

8.88

11.148

12.282

8.7

 

K2

9.258

8.058

10.35

10.218

8.01

13.338

 

K3

7.788

11.862

9.432

7.29

8.37

6.618

 

k1

1.935

1.457

1.48

1.858

2.047

1.45

 

k2

1.543

1.343

1.725

1.703

1.335

2.223

 

k3

1.298

1.977

1.572

1.215

1.395

1.103

 

R

0.637

0.634

0.245

0.643

0.712

1.12

 
  1. Specific activity was obtained from the orthogonal design experiment. Each value is the average of three independent runs of experiment
  2. K1 represents the sum of the specific activity of each factor if the factor level is 1, K2 represents the sum of the specific activity of each factor if the factor level is 2, and so K3. k1 represents the average number of K1, and so on, for k2 and k3. R represents the difference of the largest and smallest k values of each factor