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Table 2 Effect of six factors on specific activity of refolded MnP

From: Expression of a fungal manganese peroxidase in Escherichia coli: a comparison between the soluble and refolded enzymes

Experiment A
Urea
(M)
B
CaCl2
(mM)
C
Hemin
(μM)
D
Glycerol
(%)
E
GSSG
(mM)
F
pH
Specific activity
(U/mg)
1 1 1 1 1 1 1 1.63 ± 0.23
2 1 2 2 2 2 2 1.89 ± 0.59
3 1 3 3 3 3 3 0.65 ± 0.02
4 2 1 1 2 2 3 0.86 ± 0.15
5 2 2 2 3 3 1 0.86 ± 0.04
6 2 3 3 1 1 2 3.63 ± 0.19
7 3 1 2 1 3 2 2.04 ± 0.24
8 3 2 3 2 1 3 1.10 ± 0.06
9 3 3 1 3 2 1 1.01 ± 0.25
10 1 1 3 3 2 2 2.23 ± 0.39
11 1 2 1 1 3 3 1.83 ± 0.25
12 1 3 2 2 1 1 3.38 ± 0.06
13 2 1 2 3 1 3 1.02 ± 0.23
14 2 2 3 1 2 1 0.86 ± 0.07
15 2 3 1 2 3 2 2.03 ± 0.10
16 3 1 3 2 3 1 0.96 ± 0.12
17 3 2 1 3 1 2 1.52 ± 0.36
18 3 3 2 1 2 3 1.16 ± 0.09
K1 11.61 8.742 8.88 11.148 12.282 8.7  
K2 9.258 8.058 10.35 10.218 8.01 13.338  
K3 7.788 11.862 9.432 7.29 8.37 6.618  
k1 1.935 1.457 1.48 1.858 2.047 1.45  
k2 1.543 1.343 1.725 1.703 1.335 2.223  
k3 1.298 1.977 1.572 1.215 1.395 1.103  
R 0.637 0.634 0.245 0.643 0.712 1.12  
  1. Specific activity was obtained from the orthogonal design experiment. Each value is the average of three independent runs of experiment
  2. K1 represents the sum of the specific activity of each factor if the factor level is 1, K2 represents the sum of the specific activity of each factor if the factor level is 2, and so K3. k1 represents the average number of K1, and so on, for k2 and k3. R represents the difference of the largest and smallest k values of each factor
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