From: Expression of a fungal manganese peroxidase in Escherichia coli: a comparison between the soluble and refolded enzymes
Factor level
A
Urea
(M)
B
CaCl2
(mM)
C
Hemin
(μM)
D
Glycerol
(%)
E
GSSG
F
pH
1
1.0
75
5.0
10
0.5
8.0
2
1.5
100
15
0.7
8.5
3
2.0
150
25
20
9.0