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Table 1 Contents of orthogonal factors and levels L18(36)

From: Expression of a fungal manganese peroxidase in Escherichia coli: a comparison between the soluble and refolded enzymes

Factor level

A

Urea

(M)

B

CaCl2

(mM)

C

Hemin

(μM)

D

Glycerol

(%)

E

GSSG

(mM)

F

pH

1

1.0

75

5.0

10

0.5

8.0

2

1.5

100

10

15

0.7

8.5

3

2.0

150

25

20

1.0

9.0