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Table 1 Contents of orthogonal factors and levels L18(36)

From: Expression of a fungal manganese peroxidase in Escherichia coli: a comparison between the soluble and refolded enzymes

Factor level A
Urea
(M)
B
CaCl2
(mM)
C
Hemin
(μM)
D
Glycerol
(%)
E
GSSG
(mM)
F
pH
1 1.0 75 5.0 10 0.5 8.0
2 1.5 100 10 15 0.7 8.5
3 2.0 150 25 20 1.0 9.0
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