Fig. 4

The far-UV CD and absorbance spectra of refolded and soluble MnP. a The far UV-CD circular dichroism spectra of native, refolded and soluble MnP at room temperature and 0.1 mg/ml of protein concentration in 0.15 M phosphate buffer at pH 6.5. b Electronic absorption spectra of refolded and soluble MnP at room temperature. The region between 450 and 700 nm has been expanded (×5 absorbance). Enzymatic specific activities are represented in the inset