Fig. 2From: Expression of a fungal manganese peroxidase in Escherichia coli: a comparison between the soluble and refolded enzymesEffect of pH and temperature on the activity and stability of refolded and soluble MnP. And MnP activity at the beginning was considered to be 100%. Deviation values are standard deviations based on triplicate determinations. a The pH optimum of refolded and soluble MnP. The MnP activity was determined in the citrate-phosphate buffer, pH 2.2-8.0 and Tris–HCl, pH 8.6-9.0, at 25 °C. b The pH stability of refolded and soluble MnP. Refolded and soluble MnP was incubated for 6 h at 25 °C in various pH vaules in citrate-phosphate (2.2-8.0) or Tris–HCl buffer (8.6-9.0). The residual MnP activity was measured according to the method of MnP activity assay. c The temperature optimum of refolded and soluble MnP. The enzyme reaction was performed in 0.11 M sodium lactate buffer, pH 4.5 at 10–75 °C. d The temperature stability of refolded and soluble MnP. Refolded and soluble MnP were incubated for 6 h at 15–65 °C. The residual MnP activity was measured according to the method of MnP activity assayBack to article page