Table 5 Properties of primers used in gene synthesis of 96 genes encoding venom peptides. Error rate determined for the integrated HTP gene synthesis platform developed in this study
Number of genes | Number of primers in the PCR assembly | Primers lengths (nt) | Primers overlaps/gaps (nt) | Bases sequenced | Number of bases deleted, inserted or substituted | Error rate (error/kb) | Number of deletions | Number of insertions | Number of substitutions | Genes with 1 clone sequenced | Genes with 2 clones sequenced | Genes with 3 clones sequenced |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
96 | 6 | 57–60 | 20 | 23,058 | 26 | 1.13 | 20 | 1 | 5 | 77 | 17 | 2 |