Fig. 2

The efficacy of PCA-DT and PCA-DTF methodologies to generate small nucleic acids. Panel a: synthesis of gene A was performed using a vector DNA template (A1, A2 and A3) and the PCR products correspond to a 3,099 bp DNA fragment, which combines gene A sequence plus pNZY28 vector sequence. b: gene A was also assembled using different pools of overlapping oligonucleotides (B1, B2 and B3); lane C⁻ corresponds to a negative control reaction performed at the same conditions, without addition of primers. c: Effect of primer design on percentage of clones without errors. M1: NZYLadder III, M2: NZYLadder I