Table 2 Cell viability of each cryopreservation solution for both keratinocytes and fibroblasts on Day 3. Numbers are reported as mean cell number
Cell Type | 10% DMSO + 90% FCS | 5% DMSO + 95% FCS | 5% DMSO + 5% HES + 90% FCS | 10% HES + 90% FCS | 10% HES + 90% DMEM | 10% DMSO + 90% DMEM | Fresh |
|---|---|---|---|---|---|---|---|
HaCaT Suspension | 93328 | 217208 | 117635 | 88089 | 71273 | 103816 | 70383 |
HaCaT Adherent | 360294 | 436032 | 244156 | 151272 | 80313 | 376274 | 177079 |
BJ Suspension | 100002 | 130181 | 107568 | 76172 | 40912 | 131993 | 54945 |
BJ Adherent | 80448 | 168692 | 123244 | 14801 | 15238 | 189289 | 202214 |
Primary Fibroblast Suspension | 77990 | 73114 | 53455 | 37670 | 65406 | 63977 | 136237 |
Primary Fibroblast Adherent | 60997 | 58264 | 57475 | 14271 | 39625 | 23652 | 36170 |