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Table 1 Characteristics and reactivities of the generated anti-NS1 mAbs

From: Generation of monoclonal antibodies against native viral proteins using antigen-expressing mammalian cells for mouse immunization

  IFA ELISA Western blot analysis
Transfected HEK cells Transfected HEK cell lysates DENV-infected Vero E6 cell lysates
mAb Isotype D1 D4 D1 D2 D3 D4 D1 D2 D3 D4
NR1.1 IgG1, κ + - + - - - + - - -
NR1.2 IgG1, κ + - + - - - + - + -
NR1.3 IgG1, κ + - + - - - + - - -
NR1.4 IgG2a, κ + + + + + - + + + -
NR1.5 IgG2a, κ + + + - - + + - - -
NR1.6 IgG1, κ + - + - - - + - - -
NR1.7 IgG2a, κ + - + - - - + - - -
NR1.8 IgG2b, κ + + + - + + + + + +
NR4.1 IgG2a, κ - + - - - + - - - +
NR4.2 IgG2a, κ - + - - - + - - - +
NR4.3 IgG1, κ + + - - - + - - - +
NR4.4 IgG2a, κ - + - - - + - - - +
  1. The different recognition patterns of the 12 generated anti-NS1 mAbs as determined by i) IFA on the D1NS1 and D4NS1 transfected HEK cells, ii) ELISA on whole protein lysates of D1NS1-HEK - D4NS1-HEK cells and iii) Western blot analysis on whole protein lysates of DENV-infected VeroE6 cells are summarized