Generation of monoclonal antibodies against native viral proteins using antigen-expressing mammalian cells for mouse immunization

Table 1 Characteristics and reactivities of the generated anti-NS1 mAbs

		IFA		ELISA				Western blot analysis
		Transfected HEK cells		Transfected HEK cell lysates				DENV-infected Vero E6 cell lysates
mAb	Isotype	D1	D4	D1	D2	D3	D4	D1	D2	D3	D4
NR1.1	IgG1, κ	+	-	+	-	-	-	+	-	-	-
NR1.2	IgG1, κ	+	-	+	-	-	-	+	-	+	-
NR1.3	IgG1, κ	+	-	+	-	-	-	+	-	-	-
NR1.4	IgG2a, κ	+	+	+	+	+	-	+	+	+	-
NR1.5	IgG2a, κ	+	+	+	-	-	+	+	-	-	-
NR1.6	IgG1, κ	+	-	+	-	-	-	+	-	-	-
NR1.7	IgG2a, κ	+	-	+	-	-	-	+	-	-	-
NR1.8	IgG2b, κ	+	+	+	-	+	+	+	+	+	+
NR4.1	IgG2a, κ	-	+	-	-	-	+	-	-	-	+
NR4.2	IgG2a, κ	-	+	-	-	-	+	-	-	-	+
NR4.3	IgG1, κ	+	+	-	-	-	+	-	-	-	+
NR4.4	IgG2a, κ	-	+	-	-	-	+	-	-	-	+

The different recognition patterns of the 12 generated anti-NS1 mAbs as determined by i) IFA on the D1NS1 and D4NS1 transfected HEK cells, ii) ELISA on whole protein lysates of D1NS1-HEK - D4NS1-HEK cells and iii) Western blot analysis on whole protein lysates of DENV-infected VeroE6 cells are summarized

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