Fig. 6

ELISA and Western blot analysis of lysates derived from DENV-infected VeroE6 cell lines. a: NS1 relative units were determined in lysates of VeroE6 cells infected with DENV serotypes 1–4 by NS1 capture ELISA (EUROIMMUN, Luzern, Switzerland). NS1 relative units were calculated in accordance with the manufacturer’s instructions and approximate values are shown. Gels for Western blot analysis were loaded with DENV serotype 1–4 infected VeroE6 cell lysates containing 40.000 relative units of NS1, each under non-reducing conditions. The 12 generated mAbs were tested on serotype 1 (b), serotype 2 (c), serotype 3 (d) and serotype 4 (e) virus-infected cell lysates