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Fig. 5 | BMC Biotechnology

Fig. 5

From: Generation of monoclonal antibodies against native viral proteins using antigen-expressing mammalian cells for mouse immunization

Fig. 5

ELISAs of D1NS1-HEK - D4NS1-HEK cell whole protein lysates with the generated mAbs. DENV NS1 serotype-specificity of the 12 generated mAbs was assessed by capturing the NS1 protein in D1NS1 - D4NS1 HEK cell lysates using anti-hexa-His tag mAbs and detecting the protein with the anti-NS1 mAbs NR1.1 - NR1.8 and NR4.1 - NR4.4. a: While analyses with mAbs NR1.1 - NR1.3, NR1.6 and NR1.7 exhibited a high degree of D1NS1-specificity in the ELISA, mAbs NR1.4, NR1.5 and NR1.8 showed a certain degree of NS1 cross-reactivity among different DENV serotypes. b: MAbs NR4.1 - NR4.4 were highly specific for D4NS1, since they did not recognize D1NS1 - D3NS1 in the HEK cell lysates. M = molecular weight marker in kDa

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BMC Biotechnology

ISSN: 1472-6750

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