Skip to main content
Fig. 3 | BMC Biotechnology

Fig. 3

From: Generation of monoclonal antibodies against native viral proteins using antigen-expressing mammalian cells for mouse immunization

Fig. 3

ELISAs showing humoral immune responses of mice immunized with D1NS1-HEK and D4NS1-HEK cells. a and b: Mouse antisera of D1NS1-HEK (red, panel a) and D4NS1-HEK (blue, panel b) immunized mouse groups (4 mice each) were analyzed on recombinant D1NS1 and recombinant D4NS1, respectively. c and d: Serotype cross-reactive humoral immune responses of D1NS1-HEK (red, panel c) and D4NS1-HEK (blue, panel d) mouse groups were analyzed on recombinant D4NS1 and recombinant D1NS1, respectively. Blue line (panel c) and red line (panel d) = pool of D4NS1-HEK and D1NS1-HEK mouse antisera, respectively, to enable direct comparison with the level of serotype-specific immune responses. e and f: Analysis of pooled mouse sera (red = D1NS1-HEK (e), blue = D4NS1-HEK (f)) on an unrelated hexa-His tagged protein (MUL_3720) as a control for humoral responses against the hexa-His tag. Black line in all panels = pool of mouse sera before immunization (pre-bleed)

Back to article page