Fig. 1

Expression of D1NS1 and D4NS1 by transfected HEK cells. HEK cell lysates were analyzed by aqua-staining and Western blot analysis after separation on NuPAGE Novex 4–12 % Bis-Tris Gels under reducing conditions (addition of reducing agent and heating of the samples). While aqua-staining of lysates prepared from HEK-derived cell lines expressing D1NS1 (D1) and D4NS1 (D4) showed no additional band of the predicted size of the NS1 proteins, as compared to the lysates of untransfected HEK cells (−) (a), Western blot analysis using anti-hexa-His tag antibodies confirmed the expression of D1NS1 and D4NS1 by the HEK cells (b). Western blotting using anti-tubulin antibodies was performed as a control for the amount of cellular proteins of the untransfected and transfected HEK cell lysates loaded on the gels (c). M = molecular weight marker in kDa