Fig. 3
Kinetic parameters of Pyk2. The kinetic parameters of Pyk2 with respect to the substrates are shown. a PEP. b ADP. The kinetic parameters of Pyk2 with respect to PEP at a concentration of 5Â mM different effectors are shown. c FBP. d ATP. e CIT. The data points were fitted to the allosteric-sigmoidal equation using Prism software (GraphPad, USA). The S 0.5 (the substrate concentration giving one-half of the V max) and k cat values were calculated. The experiments were performed at least in three times, and the error bars represent the standard error of the regression