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Fig. 3 | BMC Biotechnology

Fig. 3

From: Improvement of alkalophilicity of an alkaline xylanase Xyn11A-LC from Bacillus sp. SN5 by random mutation and Glu135 saturation mutagenesis

Fig. 3

Locations and structural analysis of three key mutation sites. a Mutation location in the crystal structure of Xyn11A-LC (PDB accession no. 4IXL). The side chains of the catalytic residues (E93 and E183) and mutation (E135) are shown in red and green, respectively. A ‘thumb’ formed by the eight-residue loop is shown in green. b Location and mutation of E135V/R. E135, V135 and R135 are shown in green, orange, and purple, respectively. Salt bridges are represented in yellow by dashed lines

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