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Fig. 4 | BMC Biotechnology

Fig. 4

From: Novel prokaryotic expression of thioredoxin-fused insulinoma associated protein tyrosine phosphatase 2 (IA-2), its characterization and immunodiagnostic application

Fig. 4

Biochemical characterization of TrxIA-2ic. a: Size Exclusion Chromatography of affinity-purified TrxIA-2ic (solid line) and molecular weight globular calibrators (dotted line) performed on a Superdex 75 column (insert: calibration curve). The detector was set at 280 nm. From left to right, calibrator peaks correspond to human gamma-globulin (158 kDa), ovalbumin (44 kDa), horse myoglobin (17 kDa) and B12 vitamin (1.35 kDa). Arrows indicated peaks from monomeric (Ve = 9.793 mL) and dimeric TrxIA-2ic (Ve = 8.753 mL). b: TrxIA-2ic sequence. Underlined and highlighted in bold are those fragments identified by Orbitrap Mass Spectrometry considering high confidence peptides. c: TrxIA-2ic N-terminal Trx fraction, the linker peptide and the IA-2ic fragment in the C-terminal of the construction are detailed in the scheme above, and the corresponding peptide fragments identified by Orbitrap Mass Spectrometry are highlighted in the diagram below. JM: Juxtamembrane

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