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Fig. 2 | BMC Biotechnology

Fig. 2

From: Novel prokaryotic expression of thioredoxin-fused insulinoma associated protein tyrosine phosphatase 2 (IA-2), its characterization and immunodiagnostic application

Fig. 2

IA-2ic expression as a fusion protein with Trx in E. coli GI698 and GI724. a and c: SDS-PAGE (12.1 % T 6.0 % C, 1 mm, under reducing conditions, stained with Coomassie Brilliant Blue R-250), b and d: WB revealed with a rabbit polyclonal serum to thioredoxin as primary antibody. Samples: Total Cell Lysate for a and b, Intracellular Soluble Fraction for c and d. Lanes 1-4: samples from pTrxIA-2ic transformed E. coli GI698 strain; lanes 5-8: samples from pTrxIA-2ic transformed E. coli GI724 strain. Lanes 1 and 5: sample before induction (0 h); lanes 2 and 6: sample after 1.5 h of induction; lanes 3 and 7: sample after 3.0 h of induction; lanes 4 and 8: sample after 16.0 h of induction. Arrows indicate the electrophoretic mobility of TrxIA-2ic

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BMC Biotechnology

ISSN: 1472-6750

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